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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Fibronectin Monoclonal antibody (66042-1-Ig)

    WB analysis of human plasma using 66042-1-Ig (same clone as 66042-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MG-63 cells using Fibronectin Monoclonal antibody (66042-1-Ig)

    WB analysis of MG-63 using 66042-1-Ig (same clone as 66042-1-PBS)

    MG-63 cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Fibronectin Monoclonal antibody (66042-1-Ig)

    WB analysis using 66042-1-Ig (same clone as 66042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using Fibronectin Monoclonal antibody (66042-1-Ig)

    WB analysis of HuH-7 using 66042-1-Ig (same clone as 66042-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using Fibronectin Monoclonal antibody (66042-1-Ig)

    WB analysis of U2OS using 66042-1-Ig (same clone as 66042-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat serum using Fibronectin Monoclonal antibody (66042-1-Ig)

    WB analysis of rat serum using 66042-1-Ig (same clone as 66042-1-PBS)

    rat serum were subjected to SDS PAGE followed by western blot with 66042-1-Ig (Fibronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human plasma using Fibronectin Monoclonal antibody (66042-1-Ig)

    IP experiment of human plasma using 66042-1-Ig (same clone as 66042-1-PBS)

    IP result of anti-Fibronectin (IP:66042-1-Ig, 5ug; Detection:66042-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Fibronectin Monoclonal antibody (66042-1-Ig)

    IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Fibronectin Monoclonal antibody (66042-1-Ig)

    IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Fibronectin Monoclonal antibody (66042-1-Ig)

    IHC staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Fibronectin Monoclonal antibody (66042-1-Ig)

    IHC staining of human breast cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Fibronectin Monoclonal antibody (66042-1-Ig)

    IHC staining of human breast cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66042-1-Ig (Fibronectin antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Fibronectin Monoclonal antibody (66042-1-Ig)

    IF Staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Fibronectin Monoclonal antibody (66042-1-Ig)

    IF Staining of human colon cancer using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Fibronectin Monoclonal antibody (66042-1-Ig)

    IF Staining of NIH/3T3 using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Fibronectin antibody (66042-1-Ig, Clone: 1G10F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Fibronectin Monoclonal antibody (66042-1-Ig)

    IF Staining of NIH/3T3 using 66042-1-Ig (same clone as 66042-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol ) fixed NIH/3T3 cells using 66042-1-Ig(Fibronectin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66042-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Fibronectin Monoclonal antibody, PBS Only

Fibronectin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 66042-1-PBS
Clone No.1G10F9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

FN1, ED B, Cold-insoluble globulin, Cold insoluble globulin, CIG

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66042-1-PBS targets Fibronectin in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fibronectin fusion protein Ag8016 相同性解析による交差性が予測される生物種
Full Name fibronectin 1
Calculated molecular weight 2386 aa, 263 kDa
Observed molecular weight 263 kDa
GenBank accession numberBC005858
Gene Symbol Fibronectin
Gene ID (NCBI) 2335
RRIDAB_11182385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02751
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)