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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Fibulin 5 Polyclonal antibody (29992-1-AP)

    WB analysis using 29992-1-AP (same clone as 29992-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29992-1-AP (Fibulin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Fibulin 5 Polyclonal antibody (29992-1-AP)

    IHC staining of human lung cancer using 29992-1-AP (same clone as 29992-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Fibulin 5 Polyclonal antibody (29992-1-AP)

    IHC staining of human colon cancer using 29992-1-AP (same clone as 29992-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Fibulin 5 Polyclonal antibody (29992-1-AP)

    IHC staining of mouse lung using 29992-1-AP (same clone as 29992-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Fibulin 5 Polyclonal antibody (29992-1-AP)

    IHC staining of mouse colon using 29992-1-AP (same clone as 29992-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29992-1-AP (Fibulin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29992-1-PBS in a different storage buffer formulation.

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Fibulin 5 Polyclonal antibody, PBS Only

Fibulin 5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29992-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29992-1-PBS targets Fibulin 5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fibulin 5 fusion protein Ag30835 相同性解析による交差性が予測される生物種
Full Name fibulin 5
Calculated molecular weight 448 aa, 50 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC022280
Gene Symbol Fibulin 5
Gene ID (NCBI) 10516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBX5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Fibulin-5, also known as EVEC and DANCE, is an extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.