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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using Fibulin 5 Monoclonal antibody (60081-1-Ig)

    WB analysis of MCF-7 using 60081-1-Ig (same clone as 60081-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using Fibulin 5 Monoclonal antibody (60081-1-Ig)

    WB analysis of MCF-7 using 60081-1-Ig (same clone as 60081-1-PBS)

    WB result of Fibulin 5 antibody (60081-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Fibulin 5 transfected MCF-7 cells. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Fibulin 5 Monoclonal antibody (60081-1-Ig)

    WB analysis of human placenta using 60081-1-Ig (same clone as 60081-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Fibulin 5 Monoclonal antibody (60081-1-Ig)

    WB analysis of human heart using 60081-1-Ig (same clone as 60081-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using Fibulin 5 Monoclonal antibody (60081-1-Ig)

    WB analysis of rat heart using 60081-1-Ig (same clone as 60081-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using Fibulin 5 Monoclonal antibody (60081-1-Ig)

    WB analysis of pig heart using 60081-1-Ig (same clone as 60081-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Fibulin 5 Monoclonal antibody (60081-1-Ig)

    IF Staining of A549 using 60081-1-Ig (same clone as 60081-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed A549 cells using 60081-1-Ig(Fibulin 5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Fibulin 5 Monoclonal antibody, PBS Only

Fibulin 5 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 60081-1-PBS
Clone No.2A5C8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, FLJ90059, UP50, Urine p50 protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60081-1-PBS targets Fibulin 5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fibulin 5 fusion protein Ag2859 相同性解析による交差性が予測される生物種
Full Name fibulin 5
Calculated molecular weight 448 aa, 50 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC022280
Gene Symbol Fibulin 5
Gene ID (NCBI) 10516
RRIDAB_2231920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBX5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.