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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Filamin B Recombinant antibody (85202-1-RR)

    IF Staining of HeLa using 85202-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Filamin B antibody (85202-1-RR, Clone: 242275B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Filamin B Recombinant antibody (85202-1-RR)

    IF Staining of A549 using 85202-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Filamin B antibody (85202-1-RR, Clone: 242275B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293T cells using Filamin B Recombinant antibody (85202-1-RR)

    FC experiment of HEK-293T using 85202-1-RR

    1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Filamin B Recombinant antibody (85202-1-RR, Clone:242275B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Filamin B Recombinant antibody

Filamin B Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 85202-1-RR
Clone No.242275B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

FLNB, Filamin-3, Beta-filamin, Actin-binding-like protein, ABP-280 homolog

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells, A549 cells
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85202-1-RR targets Filamin B in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name filamin B, beta (actin binding protein 278)
Calculated molecular weight 278 kDa
GenBank accession numberNM_001457
Gene Symbol FLNB
Gene ID (NCBI) 2317
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75369
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FLNB, also named as FLN1L, FLN3, TABP, TAP and Fh1, belongs to the filamin family. FLNB connects cell membrane constituents to the actin cytoskeleton. It may promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. It is an anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA, FLNB may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis.

Protocols

Product Specific Protocols
IF protocol for Filamin B antibody 85202-1-RRDownload protocol
FC protocol for Filamin B antibody 85202-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols