Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 43 publications below |
| IF | See 4 publications below |
| IP | See 15 publications below |
| CoIP | See 5 publications below |
Product Information
66008-2-Ig targets DDDDK tag in WB, IF, IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | Flag Tag |
| Gene Symbol | Flag Tag |
| Gene ID (NCBI) | 8 |
| RRID | AB_2881492 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDDDDK. The structure of DDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, theDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This DDDDK-tag antibody can be used to detect the DDDDK tagged protein with pCMV-DDDDK, p3XFLAG-CMV, pcDNA3.1-DDDDK, pef-NEO-DDDDK, DDDDK-RIG-I, pLenti6-3XDDDDK and some other vectors.
Publications
| Species | Application | Title |
|---|---|---|
Circ Res Unspliced XBP1 Confers VSMC Homeostasis and Prevents Aortic Aneurysm Formation via FoxO4 Interaction. | ||
Nat Commun Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet. | ||
Nat Commun Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia. | ||
Nucleic Acids Res The RNA binding protein EWS is broadly involved in the regulation of pri-miRNA processing in mammalian cells. | ||
Hepatology Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients. |