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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Flightless I Polyclonal antibody, PBS Only

Flightless I Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27729-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Flightless I Polyclonal antibody (27729-1-AP)

    WB analysis using 27729-1-AP (same clone as 27729-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27729-1-AP (Flightless I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27729-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Flightless I Polyclonal antibody (27729-1-AP)

    WB analysis using 27729-1-AP (same clone as 27729-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27729-1-AP (Flightless I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27729-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Flightless I Polyclonal antibody (27729-1-AP)

    IHC staining of human colon using 27729-1-AP (same clone as 27729-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27729-1-AP (Flightless I antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27729-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Flightless I Polyclonal antibody (27729-1-AP)

    IHC staining of human colon using 27729-1-AP (same clone as 27729-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27729-1-AP (Flightless I antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27729-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Flightless I Polyclonal antibody (27729-1-AP)

    IF Staining of A431 using 27729-1-AP (same clone as 27729-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Flightless I antibody (27729-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27729-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27729-1-PBS targets Flightless I in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Flightless I fusion protein Ag26865 相同性解析による交差性が予測される生物種
Full Name flightless I homolog (Drosophila)
Calculated molecular weight 1269 aa, 145 kDa
Observed molecular weight 145-150 kDa
GenBank accession numberBC025300
Gene symbol Flightless I
Gene ID (NCBI) 2314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).