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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Flotillin 1 Monoclonal antibody

Flotillin 1 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D6C6

Cat no : 67968-1-Ig

Synonyms

FLOT1, Flotillin-1, 1D6C6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Flotillin 1 Monoclonal antibody (67968-1-Ig)

    WB analysis using 67968-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Flotillin 1 Monoclonal antibody (67968-1-Ig)

    IHC staining of human ovary tumor using 67968-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Flotillin 1 Monoclonal antibody (67968-1-Ig)

    IHC staining of human stomach cancer using 67968-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Flotillin 1 Monoclonal antibody (67968-1-Ig)

    IHC staining of human stomach cancer using 67968-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Flotillin 1 Monoclonal antibody (67968-1-Ig)

    IHC staining of human ovary tumor using 67968-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67968-1-Ig (Flotillin 1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of Raji cells using Flotillin 1 Monoclonal antibody (67968-1-Ig)

    FC experiment of Raji using 67968-1-Ig

    1x10^6 Raji cells were intracellularly stained with 0.2 ug Flotillin 1 Monoclonal antibody (67968-1-Ig, Clone:1D6C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inLNCaP cells, Raji cells, Jurkat cells, MOLT-4 cells, human peripheral blood platelets, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive FC (Intra) detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67968-1-Ig targets Flotillin 1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Flotillin 1 fusion protein Ag8694 相同性解析による交差性が予測される生物種
Full Name flotillin 1
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC001146
Gene symbol Flotillin 1
Gene ID (NCBI) 10211
RRIDAB_2918719
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Protocols

Product Specific Protocols
WB protocol for Flotillin 1 antibody 67968-1-IgDownload protocol
IHC protocol for Flotillin 1 antibody 67968-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols