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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Flotillin 2 Polyclonal antibody, PBS Only

Flotillin 2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28208-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Flotillin 2 Polyclonal antibody (28208-1-AP)

    WB analysis using 28208-1-AP (same clone as 28208-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28208-1-AP (Flotillin 2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28208-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Flotillin 2 Polyclonal antibody (28208-1-AP)

    IHC staining of human breast cancer using 28208-1-AP (same clone as 28208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28208-1-AP (Flotillin 2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28208-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Flotillin 2 Polyclonal antibody (28208-1-AP)

    IF Staining of HeLa using 28208-1-AP (same clone as 28208-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Flotillin 2 antibody (28208-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28208-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28208-1-PBS targets Flotillin 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Flotillin 2 fusion protein Ag28308 相同性解析による交差性が予測される生物種
Full Name flotillin 2
Calculated molecular weight 379 aa, 42 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC017292
Gene symbol Flotillin 2
Gene ID (NCBI) 2319
RRIDAB_2881089
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.