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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Follistatin Polyclonal antibody

Follistatin Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27350-1-AP

Synonyms

Activin binding protein, follistatin, FS, FST

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Follistatin Polyclonal antibody (27350-1-AP)

    WB analysis using 27350-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27350-1-AP (Follistatin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Follistatin Polyclonal antibody (27350-1-AP)

    WB analysis using 27350-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27350-1-AP (Follistatin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Follistatin Polyclonal antibody (27350-1-AP)

    IF Staining of HeLa using 27350-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Follistatin antibody (27350-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, Calu-3 cells, HeLa cells, U2OS cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

27350-1-AP targets Follistatin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Follistatin fusion protein Ag26401 相同性解析による交差性が予測される生物種
Full Name follistatin
Calculated molecular weight 33 aa, 7 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC004107
Gene symbol Follistatin
Gene ID (NCBI) 10468
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Follistatin (FST) is a member of the tissue growth factor β family and is a secreted glycoprotein that antagonizes many members of the family, including activin A, growth differentiation factor11 , and myostatin. It binds activin A with high affinity and whose expression can be induced by activin A and several other pro-inflammatory cytokines. Activin A-follistatin complexes are biologically inactive and bind to cell surface heparan sulphate-containing proteoglycans for internalisation and degradation. It has also been found to play a significant role in the management of skeletal muscle size and mass. It also has important roles in early embryonic development, differentiation of ovarian granulosa cells, liver fibrosis and polycystic ovarian syndrome.

Protocols

Product Specific Protocols
WB protocol for Follistatin antibody 27350-1-APDownload protocol
IF protocol for Follistatin antibody 27350-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratIHC

Hypertens Res

Follistatin lowers blood pressure and improves vascular structure and function in essential and secondary hypertension

Authors - Ann Kuganathan
View Article