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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Foxp3 / Transcription Factor Fix/Perm Concentrate (4X)

Concentration:

4x

Application:

FC

Cat no : PF00011-A

Validation Data Gallery

  • 1X10^6 mouse splenocytes were surface stained with CoraLite488-conjugated Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5) and then fixed with 1X Transcription Factor Fix/Perm buffer (PF00011-A) and permeabilized with 1X Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with CoraLite647-conjugated mouse IgG1 isotype control or 5 ul CoraLite647-conjugated Anti-Mouse Foxp3 (CL647-65089, Clone: 3G3).

    1X10^6 mouse splenocytes were surface stained with CoraLite488-conjugated Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5) and then fixed with 1X Transcription Factor Fix/Perm buffer (PF00011-A) and permeabilized with 1X Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with CoraLite647-conjugated mouse IgG1 isotype control or 5 ul CoraLite647-conjugated Anti-Mouse Foxp3 (CL647-65089, Clone: 3G3).

Product Information

Foxp3 / Transcription Factor Fix/Perm Concentrate (4X): provided in a concentrated soltion which, when diluted with Foxp3 / Transcription Factor Fix/Perm Diluent (1X) provide best results during intracellular staining of transcription factors using fluorescently conjugated antibodies.

Storage

4°C

Expiration Date

6 months after receiving.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Harshit (Verified Customer) (07-22-2022)

I was facing issues with intracellular staining of flow cytometry, after adding the kit my problem was solved it gives me better intracellular staining specially the transcription factor.

  • Applications: Flow Cytometry
  • Cell Tissue Type: Immune cells