- Featured Product
- KD/KO Validated
G-CSF Polyclonal antibody
G-CSF Polyclonal Antibody for WB, ELISA, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA, FC (Intra)
Conjugate
Unconjugated
Cat no : 17185-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MOLT-4 cells, MCF-7 cells, K-562 cells |
| Positive FC (Intra) detected in | K-562 cells |
| Positive FC detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 2 publications below |
Product Information
17185-1-AP targets G-CSF in WB, IHC, ELISA, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | G-CSF fusion protein Ag10968 相同性解析による交差性が予測される生物種 |
| Full Name | colony stimulating factor 3 (granulocyte) |
| Calculated molecular weight | 200 aa, 22 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC033245 |
| Gene symbol | G-CSF |
| Gene ID (NCBI) | 1440 |
| RRID | AB_2878357 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for G-CSF antibody 17185-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Immunol Granulocyte colony stimulating factor (G-CSF) regulates neutrophils infiltration and periodontal tissue destruction in an experimental periodontitis. | ||
Int J Mol Sci A NOTCH1 Mutation Found in a Newly Established Ovarian Cancer Cell Line (FDOVL) Promotes Lymph Node Metastasis in Ovarian Cancer
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