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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using G3BP1 Monoclonal antibody (66486-1-Ig)

    WB analysis using 66486-1-Ig (same clone as 66486-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using G3BP1 Monoclonal antibody (66486-1-Ig)

    WB analysis using 66486-1-Ig (same clone as 66486-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using G3BP1 Monoclonal antibody (66486-1-Ig)

    WB analysis of RAW 264.7 using 66486-1-Ig (same clone as 66486-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using G3BP1 Monoclonal antibody (66486-1-Ig)

    WB analysis of pig brain using 66486-1-Ig (same clone as 66486-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using G3BP1 Monoclonal antibody (66486-1-Ig)

    WB analysis of mouse brain using 66486-1-Ig (same clone as 66486-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using G3BP1 Monoclonal antibody (66486-1-Ig)

    IHC staining of human testis using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using G3BP1 Monoclonal antibody (66486-1-Ig)

    IHC staining of human testis using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using G3BP1 Monoclonal antibody (66486-1-Ig)

    IHC staining of rat brain using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66486-1-Ig (G3BP1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using G3BP1 Monoclonal antibody (66486-1-Ig)

    IHC staining of rat brain using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66486-1-Ig (G3BP1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using G3BP1 Monoclonal antibody (66486-1-Ig)

    IHC staining of human lymphoma using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using G3BP1 Monoclonal antibody (66486-1-Ig)

    IHC staining of human lymphoma using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using G3BP1 Monoclonal antibody (66486-1-Ig)

    IHC staining of human colon using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using G3BP1 Monoclonal antibody (66486-1-Ig)

    IHC staining of human colon using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using G3BP1 Monoclonal antibody (66486-1-Ig)

    IF Staining of HeLa using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (66486-1-Ig, Clone: 1E4A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

G3BP1 Monoclonal antibody, PBS Only

G3BP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66486-1-PBS
Clone No.1E4A2

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66486-1-PBS targets G3BP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen G3BP1 fusion protein Ag3728 相同性解析による交差性が予測される生物種
Full Name GTPase activating protein (SH3 domain) binding protein 1
Calculated molecular weight 466 aa, 52 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC006997
Gene Symbol G3BP1
Gene ID (NCBI) 10146
RRIDAB_2819031
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13283
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.