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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

G3BP2 Monoclonal antibody

G3BP2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E5G3

Cat no : 68580-1-Ig

Synonyms

G3BP 2, G3BP2, KIAA0660

Validation Data Gallery

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  • Western Blot (WB) analysis of SCaBER cells using G3BP2 Monoclonal antibody (68580-1-Ig)

    WB analysis of SCaBER using 68580-1-Ig

    SCaBER cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using G3BP2 Monoclonal antibody (68580-1-Ig)

    WB analysis using 68580-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of A549 cells using G3BP2 Monoclonal antibody (68580-1-Ig)

    WB analysis of A549 using 68580-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using G3BP2 Monoclonal antibody (68580-1-Ig)

    WB analysis of rat brain using 68580-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HCT 116 cells using G3BP2 Monoclonal antibody (68580-1-Ig)

    WB analysis of HCT 116 using 68580-1-Ig

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using G3BP2 Monoclonal antibody (68580-1-Ig)

    WB analysis using 68580-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68580-1-Ig (G3BP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using G3BP2 Monoclonal antibody (68580-1-Ig)

    IF Staining of HeLa using 68580-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (68580-1-Ig, Clone: 2E5G3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, SCaBER cells, rat brain tissue, HCT 116 cells, HepG2 cells, A549 cells, HT-29 cells, K-562 cells, MOLT-4 cells
Positive IF/ICC detected insodium arsenite treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68580-1-Ig targets G3BP2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen G3BP2 fusion protein Ag9222 相同性解析による交差性が予測される生物種
Full Name GTPase activating protein (SH3 domain) binding protein 2
Calculated molecular weight482aa,54 kDa; 449aa,51 kDa
Observed molecular weight54 kDa
GenBank accession numberBC011731
Gene symbol G3BP2
Gene ID (NCBI) 9908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

Protocols

Product Specific Protocols
WB protocol for G3BP2 antibody 68580-1-IgDownload protocol
IF protocol for G3BP2 antibody 68580-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols