Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 25413-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 25413-1-AP
WB result of G6PD antibody (25413-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G6PD transfected HeLa cells.
WB analysis of HeLa using 25413-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 25413-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 25413-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 25413-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25413-1-AP (G6PD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 25413-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 25413-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 25413-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 25413-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25413-1-AP (G6PD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 25413-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using G6PD antibody (25413-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

G6PD Polyclonal antibody

G6PD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25413-1-AP

Publications(43)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Glucose-6-phosphate 1-dehydrogenase, G6PD1, G6P, EC:1.1.1.49

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (43)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF-7 cells, HeLa cells, HEK-293 cells
Positive IHC detected in	human liver cancer tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 36 publications below
IHC	See 8 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

25413-1-AP targets G6PD in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, rat, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	G6PD fusion protein Ag21862 相同性解析による交差性が予測される生物種
Full Name	glucose-6-phosphate dehydrogenase
Calculated molecular weight	59 kDa
Observed molecular weight	59 kDa
GenBank accession number	BC000337
Gene Symbol	G6PD
Gene ID (NCBI)	2539
RRID	AB_2880066
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P11413
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for G6PD antibody 25413-1-AP	Download protocol
IHC protocol for G6PD antibody 25413-1-AP	Download protocol
IF protocol for G6PD antibody 25413-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Sci Adv G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis. Authors - Yang Zhang View Article
human	WB, IP	Nat Commun Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway. Authors - Xiaoyu Ma View Article
human	WB	Acta Pharmacol Sin Targeting proteasomal deubiquitinases USP14 and UCHL5 with b-AP15 reduces 5-fluorouracil resistance in colorectal cancer cells Authors - Wa Ding View Article
human	WB	Phytomedicine Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells Authors - Maolei Xu View Article
human	WB	Phytomedicine Tiliroside targets TBK1 to induce ferroptosis and sensitize hepatocellular carcinoma to sorafenib Authors - Chen Yang View Article
human,mouse	WB,IHC	Oxid Med Cell Longev Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo. Authors - Wen-Chih Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 25413-1-AP

WB analysis of HeLa using 25413-1-AP

WB analysis of HeLa using 25413-1-AP

WB analysis of MCF-7 using 25413-1-AP

WB analysis of HeLa using 25413-1-AP

WB analysis of MCF-7 using 25413-1-AP

IHC staining of human liver cancer using 25413-1-AP

IHC staining of human liver cancer using 25413-1-AP

IHC staining of human ovary tumor using 25413-1-AP

IHC staining of human ovary tumor using 25413-1-AP

IF Staining of HeLa using 25413-1-AP

G6PD Polyclonal antibody

G6PD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis.

Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.

Targeting proteasomal deubiquitinases USP14 and UCHL5 with b-AP15 reduces 5-fluorouracil resistance in colorectal cancer cells

Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells

Tiliroside targets TBK1 to induce ferroptosis and sensitize hepatocellular carcinoma to sorafenib

Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo.