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  • Featured Product
  • KD/KO Validated

G6PD Monoclonal antibody, PBS Only

G6PD Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A7B12

Cat no : 66373-1-PBS

Synonyms

G6P, G6PD, G6PD1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using G6PD Monoclonal antibody (66373-1-Ig)

    WB analysis of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

    WB result of G6PD antibody (66373-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G6PD transfected HeLa cells. This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using G6PD Monoclonal antibody (66373-1-Ig)

    WB analysis of HepG2 using 66373-1-Ig (same clone as 66373-1-PBS)

    Several cell lines were subjected to SDS PAGE followed by western blot with 66373-1-Ig (G6PD Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat spleen tissue using G6PD Monoclonal antibody (66373-1-Ig)

    WB analysis of rat spleen using 66373-1-Ig (same clone as 66373-1-PBS)

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 66373-1-Ig (G6PD Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using G6PD Monoclonal antibody (66373-1-Ig)

    IHC staining of human liver cancer using 66373-1-Ig (same clone as 66373-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66373-1-Ig (G6PD Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using G6PD Monoclonal antibody (66373-1-Ig)

    IHC staining of human liver cancer using 66373-1-Ig (same clone as 66373-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66373-1-Ig (G6PD Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using G6PD Monoclonal antibody (66373-1-Ig)

    IF Staining of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66373-1-Ig(G6PD antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using G6PD Monoclonal antibody (66373-1-Ig)

    IF Staining of HeLa using 66373-1-Ig (same clone as 66373-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66373-1-Ig (G6PD antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66373-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66373-1-PBS targets G6PD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen G6PD fusion protein Ag21862 相同性解析による交差性が予測される生物種
Full Name glucose-6-phosphate dehydrogenase
Calculated molecular weight 59 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC000337
Gene symbol G6PD
Gene ID (NCBI) 2539
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

G6PD(Glucose-6-phosphate 1-dehydrogenase) belongs to the glucose-6-phosphate dehydrogenase family. It is a housekeeping enzyme encoded in mammals by an X-linked gene. G6PD has important functions in intermediary metabolism because it catalyzes the first step in the pentose phosphate pathway and provides reductive potential in the form of NADPH.(PMID:7489710). This protein has 3 isoforms produced by alternative splicing.