GAA Polyclonal antibody
GAA Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 14367-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | DU 145 cells, mouse liver tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human pancreas cancer tissue, mouse liver tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 4 publications below |
IHC | See 1 publications below |
IF | See 5 publications below |
Product Information
14367-1-AP targets GAA in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GAA fusion protein Ag5690 相同性解析による交差性が予測される生物種 |
Full Name | glucosidase, alpha; acid |
Calculated molecular weight | 105 kDa |
Observed molecular weight | 70-76 kDa |
GenBank accession number | BC040431 |
Gene symbol | GAA |
Gene ID (NCBI) | 2548 |
RRID | AB_2107667 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GAA(Alpha-1,4-glucosidase) is a lysosomal enzyme involved in the degradation of glycogen within cellular vacuoles. After translation, GAA undergoes proteolytic processing to form two lengths of lysosomal a-glucosidase, and both N-terminal and C-terminal processing occur. The full sequence can be further processed into a mature form. The 105 kDa GAA can be cleaved into 70 kDa or 76 kDa form.
Protocols
Product Specific Protocols | |
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WB protocol for GAA antibody 14367-1-AP | Download protocol |
IHC protocol for GAA antibody 14367-1-AP | Download protocol |
IP protocol for GAA antibody 14367-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Spatial metabolomics reveals glycogen as an actionable target for pulmonary fibrosis | ||
Redox Biol Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke | ||
J Med Chem 5-C-Branched Deoxynojirimycin: Strategy for Designing a 1-Deoxynojirimycin-Based Pharmacological Chaperone with a Nanomolar Affinity for Pompe Disease. | ||
Org Biomol Chem Introduction of C-alkyl branches to L-iminosugars changes their active site binding orientation | ||
DNA Cell Biol Disruption of the gaa Gene in Zebrafish Fails to Generate the Phenotype of Classical Pompe Disease. | ||
Curr Res Physiol Glycophagy is involved in cardiac glycogen regulation in response to exercise |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH CHRISTINE (Verified Customer) (09-27-2019) | will continue to order here in the future.
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