Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | DU 145 cells, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human pancreas cancer tissue, mouse liver tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 5 publications below |
Product Information
14367-1-AP targets GAA in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GAA fusion protein Ag5690 相同性解析による交差性が予測される生物種 |
| Full Name | glucosidase, alpha; acid |
| Calculated molecular weight | 105 kDa |
| Observed molecular weight | 70-76 kDa |
| GenBank accession number | BC040431 |
| Gene Symbol | GAA |
| Gene ID (NCBI) | 2548 |
| RRID | AB_2107667 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10253 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GAA(Alpha-1,4-glucosidase) is a lysosomal enzyme involved in the degradation of glycogen within cellular vacuoles. After translation, GAA undergoes proteolytic processing to form two lengths of lysosomal a-glucosidase, and both N-terminal and C-terminal processing occur. The full sequence can be further processed into a mature form. The 105 kDa GAA can be cleaved into 70 kDa or 76 kDa form.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GAA antibody 14367-1-AP | Download protocol |
| IHC protocol for GAA antibody 14367-1-AP | Download protocol |
| IP protocol for GAA antibody 14367-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Spatial metabolomics reveals glycogen as an actionable target for pulmonary fibrosis | ||
Redox Biol Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke | ||
J Med Chem 5-C-Branched Deoxynojirimycin: Strategy for Designing a 1-Deoxynojirimycin-Based Pharmacological Chaperone with a Nanomolar Affinity for Pompe Disease. | ||
Org Biomol Chem Introduction of C-alkyl branches to L-iminosugars changes their active site binding orientation | ||
DNA Cell Biol Disruption of the gaa Gene in Zebrafish Fails to Generate the Phenotype of Classical Pompe Disease. | ||
Curr Res Physiol Glycophagy is involved in cardiac glycogen regulation in response to exercise |