GAA Polyclonal antibody
GAA Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 29993-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | DU 145 cells, LNCaP cells, PC-13 cells |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
29993-1-AP targets GAA in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Reactivity | Human, Mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GAA fusion protein Ag28970 相同性解析による交差性が予測される生物種 |
Full Name | glucosidase, alpha; acid |
Calculated molecular weight | 105 kDa |
Observed molecular weight | 110 kDa |
GenBank accession number | BC040431 |
Gene symbol | GAA |
Gene ID (NCBI) | 2548 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GAA (Alpha-1,4-glucosidase) is a lysosomal enzyme involved in the degradation of glycogen within cellular vacuoles. After translation, GAA undergoes proteolytic processing to form two lengths of lysosomal α-glucosidase, and both N-terminal and C-terminal processing occur. Typically, GAA is synthesized as an immature glycoprotein precursor (110 kDa) in the endoplasmic reticulum and undergoes a seriesof proteolytic and N-glycan processing events to yield an intermediate (95 kDa) and two lysosomal (76 and 70 kDa) isoforms. This antibody can detect the 110 kDa precursor form.
Protocols
Product Specific Protocols | |
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WB protocol for GAA antibody 29993-1-AP | Download protocol |
IHC protocol for GAA antibody 29993-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |