• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GAA Polyclonal antibody (29993-1-AP)

    WB analysis using 29993-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29993-1-AP (GAA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GAA Polyclonal antibody (29993-1-AP)

    IHC staining of mouse liver using 29993-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29993-1-AP (GAA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GAA Polyclonal antibody (29993-1-AP)

    IHC staining of mouse liver using 29993-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29993-1-AP (GAA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

GAA Polyclonal antibody

GAA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29993-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Synonyms

Acid maltase, Aglucosidase alfa, GAA, glucosidase, alpha; acid, LYAG, Lysosomal alpha glucosidase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDU 145 cells, LNCaP cells, PC-13 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29993-1-AP targets GAA in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAA fusion protein Ag28970 相同性解析による交差性が予測される生物種
Full Name glucosidase, alpha; acid
Calculated molecular weight 105 kDa
Observed molecular weight110 kDa
GenBank accession numberBC040431
Gene Symbol GAA
Gene ID (NCBI) 2548
RRIDAB_2923627
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10253
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GAA (Alpha-1,4-glucosidase) is a lysosomal enzyme involved in the degradation of glycogen within cellular vacuoles. After translation, GAA undergoes proteolytic processing to form two lengths of lysosomal α-glucosidase, and both N-terminal and C-terminal processing occur. Typically, GAA is synthesized as an immature glycoprotein precursor (110 kDa) in the endoplasmic reticulum and undergoes a seriesof proteolytic and N-glycan processing events to yield an intermediate (95 kDa) and two lysosomal (76 and 70 kDa) isoforms. This antibody can detect the 110 kDa precursor form.

Protocols

Product Specific Protocols
WB protocol for GAA antibody 29993-1-APDownload protocol
IHC protocol for GAA antibody 29993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols