• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GAB1 Polyclonal antibody

GAB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26200-1-AP

Synonyms

Growth factor receptor bound protein 2-associated protein 1, GRB2-associated-binding protein 1, GRB2-associated binder 1, GRB2 associated binder 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GAB1 Polyclonal antibody (26200-1-AP)

    WB analysis using 26200-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26200-1-AP (GAB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GAB1 Polyclonal antibody (26200-1-AP)

    WB analysis of HEK-293 using 26200-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26200-1-AP (GAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GAB1 Polyclonal antibody (26200-1-AP)

    IHC staining of mouse brain using 26200-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26200-1-AP (GAB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GAB1 Polyclonal antibody (26200-1-AP)

    IHC staining of mouse brain using 26200-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26200-1-AP (GAB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆1/31（金）までお得に買える！ >> IHC・ELISA・IPキット最大30%OFFキャンペーン

Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, C6 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26200-1-AP targets GAB1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAB1 fusion protein Ag24395 相同性解析による交差性が予測される生物種
Full Name GRB2-associated binding protein 1
Calculated molecular weight 724 aa, 80 kDa
Observed molecular weight110 kDa
GenBank accession numberBC064848
Gene symbol GAB1
Gene ID (NCBI) 2549
RRIDAB_2918101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GAB1 antibody 26200-1-APDownload protocol
IHC protocol for GAB1 antibody 26200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
View Article
humanWB,IF

Toxicol Appl Pharmacol

Hexachlorophene, a selective SHP2 inhibitor, suppresses proliferation and metastasis of KRAS-mutant NSCLC cells by inhibiting RAS/MEK/ERK and PI3K/AKT signaling pathways.

Authors - Nai-Jie Fu
View Article