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KD/KO Validated

GAB2 Polyclonal antibody

Name: GAB2 Polyclonal antibody
Brand: Proteintech
SKU: 22549-1-AP

GAB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22549-1-AP

Synonyms

GAB2, GRB2 associated binder 2, KIAA0571, pp100

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of K-562 using 22549-1-AP
WB result of GAB2 antibody (22549-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GAB2 transfected K-562 cells.
WB analysis using 22549-1-AP
K-562 cells lysates were subjected to SDS PAGE followed by western blot with 22549-1-AP (GAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse spleen using 22549-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 22549-1-AP (GAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 22549-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 22549-1-AP (GAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of K-562 using 22549-1-AP
Immunofluorescent analysis of (4% PFA) fixed K-562 cells using GAB2 antibody (22549-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Cat No. 22549-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	K-562 cells
Positive IHC detected in	mouse spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	K-562 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

22549-1-AP targets GAB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GAB2 fusion protein Ag18192 相同性解析による交差性が予測される生物種
Full Name	GRB2-associated binding protein 2
Calculated molecular weight	676 aa, 74 kDa
Observed molecular weight	74-90 kDa
GenBank accession number	BC131711
Gene symbol	GAB2
Gene ID (NCBI)	9846
RRID	AB_2879119
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for GAB2 antibody 22549-1-AP	Download protocol
IHC protocol for GAB2 antibody 22549-1-AP	Download protocol
IF protocol for GAB2 antibody 22549-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. Authors - Hongwei Chu View Article
human	WB	Am J Transl Res Silencing of lncRNA HOXA11-AS inhibits cell migration, invasion, proliferation, and promotes apoptosis in human glioma cells via upregulating microRNA-125a: in vitro and in vivo studies. Authors - Jianxin Jiang View Article
mouse	IHC	Oncol Rep MicroRNA‑218 inhibits tumor angiogenesis of human renal cell carcinoma by targeting GAB2. Authors - Lijun Mu View Article
human	WB	Front Physiol Enhanced Autophagy in GAB1-Deficient Vascular Endothelial Cells Is Responsible for Atherosclerosis Progression. Authors - Xin Qian View Article
human,mouse	WB,IF	Int J Mol Med Gab2 promotes the growth of colorectal cancer by regulating the M2 polarization of tumor‑associated macrophages Authors - Xuehan Gao View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GAB2 Polyclonal antibody

GAB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of K-562 using 22549-1-AP

WB analysis using 22549-1-AP

IHC staining of mouse spleen using 22549-1-AP

IHC staining of mouse spleen using 22549-1-AP

IF Staining of K-562 using 22549-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Silencing of lncRNA HOXA11-AS inhibits cell migration, invasion, proliferation, and promotes apoptosis in human glioma cells via upregulating microRNA-125a: in vitro and in vivo studies.

MicroRNA‑218 inhibits tumor angiogenesis of human renal cell carcinoma by targeting GAB2.

Enhanced Autophagy in GAB1-Deficient Vascular Endothelial Cells Is Responsible for Atherosclerosis Progression.

Gab2 promotes the growth of colorectal cancer by regulating the M2 polarization of tumor‑associated macrophages