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  • Featured Product
  • KD/KO Validated

GAB2 Polyclonal antibody, PBS Only

GAB2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29272-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAB2 Polyclonal antibody (29272-1-AP)

    WB analysis using 29272-1-AP (same clone as 29272-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 29272-1-AP (GAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using GAB2 Polyclonal antibody (29272-1-AP)

    WB analysis of K-562 using 29272-1-AP (same clone as 29272-1-PBS)

    WB result of GAB2 antibody (29272-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GAB2 transfected K-562 cells. This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GAB2 Polyclonal antibody (29272-1-AP)

    IHC staining of human colon cancer using 29272-1-AP (same clone as 29272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29272-1-AP (GAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GAB2 Polyclonal antibody (29272-1-AP)

    IHC staining of human colon cancer using 29272-1-AP (same clone as 29272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29272-1-AP (GAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GAB2 Polyclonal antibody (29272-1-AP)

    IHC staining of human liver cancer using 29272-1-AP (same clone as 29272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29272-1-AP (GAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GAB2 Polyclonal antibody (29272-1-AP)

    IHC staining of human liver cancer using 29272-1-AP (same clone as 29272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 29272-1-AP (GAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29272-1-PBS targets GAB2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAB2 fusion protein Ag30347 相同性解析による交差性が予測される生物種
Full Name GRB2-associated binding protein 2
Calculated molecular weight 676 aa, 74 kDa
Observed molecular weight 74-90 kDa
GenBank accession numberBC131711
Gene symbol GAB2
Gene ID (NCBI) 9846
RRIDAB_2918265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Growth factor receptor-bound protein 2 (GAB2), an adaptor protein, belongs to the Gab family and is involved in various biologic processes. Gab2 mainly mediates phosphatidylinositol 3-kinase (PI3K) and extracellular signal-regulated kinase (ERK) signaling pathways. Furthermore, Gab2 governs tumorigenic signaling and is a novel potential therapeutic target in leukemia, breast cancer, ovarian cancer, and melanoma. Gab2 is the major 98-kDa phosphoprotein associated with SHP-2, PI 3-kinase, and Grb2 in normal human lymphocytes. (PMID: 32269703, PMID: 28842424, PMID: 28420432, PMID: 10849428)