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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GAB4 Polyclonal antibody

GAB4 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 20087-1-AP

Synonyms

GRB2-associated binder 4, GRB2-associated binder 2-like, GRB2 associated binder 4, GRB2 associated binder 2 like, GAB2-like

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using GAB4 Polyclonal antibody (20087-1-AP)

    IHC staining of mouse testis using 20087-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20087-1-AP (GAB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GAB4 Polyclonal antibody (20087-1-AP)

    IHC staining of mouse testis using 20087-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20087-1-AP (GAB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using GAB4 Polyclonal antibody (20087-1-AP)

    IF Staining of mouse testis using 20087-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using GAB4 antibody (20087-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using GAB4 Polyclonal antibody (20087-1-AP)

    IF Staining of mouse testis using 20087-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using GAB4 antibody (20087-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20087-1-AP targets GAB4 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name GRB2-associated binding protein family, member 4
Calculated molecular weight 62 kDa
GenBank accession numberNM_001037814
Gene symbol GAB4
Gene ID (NCBI) 128954
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GAB4, also named as GAB2-like, belongs to the GAB family. The antibody has no cross reaction to GAB2.

Protocols

Product Specific Protocols
IHC protocol for GAB4 antibody 20087-1-APDownload protocol
IF protocol for GAB4 antibody 20087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols