Validation Data Gallery

Filter: AllWBIPIF/ICCFC (Intra)

WB analysis using 18723-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 18723-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 18723-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 18723-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 18723-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 18723-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 18723-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 18723-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 18723-1-AP (GABARAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 18723-1-AP
IP result of anti-GABARAP (IP:18723-1-AP, 4ug; Detection:18723-1-AP 1:2000) with HeLa cells lysate 1200 ug.
IF Staining of HepG2 using 18723-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells and untreated HepG2 cells using GABARAP antibody (18723-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 18723-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed Starvation treated HepG2 cells and untreated HepG2 cells using 18723-1-AP (GABARAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 18723-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human GABARAP (18723-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

GABARAP Polyclonal antibody

GABARAP Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 18723-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

MM46, HT004, Gamma-aminobutyric acid receptor-associated protein, GABA(A) receptor-associated protein, FLC3B

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (25)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, mouse brain tissue, human placenta tissue, mouse pancreas tissue, human heart tissue, mouse skeletal muscle tissue, HuH-7 cells, SH-SY5Y cells, RAW 264.7 cells, mouse liver tissue, rat brain tissue
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	HepG2 cells, Starvation treated HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 20 publications below
IF	See 5 publications below
CoIP	See 5 publications below

Product Information

18723-1-AP targets GABARAP in WB, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	GABA(A) receptor-associated protein
Calculated molecular weight	14 kDa
Observed molecular weight	14-18 kDa
GenBank accession number	BC106748
Gene Symbol	GABARAP
Gene ID (NCBI)	11337
RRID	AB_10603365
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95166
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.

Protocols

Product Specific Protocols
WB protocol for GABARAP antibody 18723-1-AP	Download protocol
IF protocol for GABARAP antibody 18723-1-AP	Download protocol
IP protocol for GABARAP antibody 18723-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation. Authors - Linyu Sun View Article
human	WB	Autophagy ATF3 -activated accelerating effect of LINC00941/lncIAPF on fibroblast-to-myofibroblast differentiation by blocking autophagy depending on ELAVL1/HuR in pulmonary fibrosis Authors - Jinjin Zhang KD Validated View Article
human	WB	Autophagy ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1 Authors - Weihong Xie KO Validated View Article
	IF	Nat Commun PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection. Authors - Shifan Wang View Article
human	WB	Autophagy ATF4 links ER stress with reticulophagy in glioblastoma cells. Authors - Svenja Zielke View Article
mouse	WB	Autophagy ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8. Authors - Mads Skytte Rasmussen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 18723-1-AP

WB analysis of human heart using 18723-1-AP

WB analysis of mouse skeletal muscle using 18723-1-AP

WB analysis of mouse brain using 18723-1-AP

WB analysis of human placenta using 18723-1-AP

WB analysis of mouse pancreas using 18723-1-AP

WB analysis using 18723-1-AP

WB analysis of A549 using 18723-1-AP

IP experiment of HeLa using 18723-1-AP

IF Staining of HepG2 using 18723-1-AP

IF Staining of HepG2 using 18723-1-AP

FC experiment of HepG2 using 18723-1-AP

GABARAP Polyclonal antibody

GABARAP Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation.

ATF3 -activated accelerating effect of LINC00941/lncIAPF on fibroblast-to-myofibroblast differentiation by blocking autophagy depending on ELAVL1/HuR in pulmonary fibrosis

ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1

PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection.

ATF4 links ER stress with reticulophagy in glioblastoma cells.

ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8.