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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GABARAPL1-Specific Polyclonal antibody

GABARAPL1-Specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 18721-1-AP

Synonyms

GABARAPL1, GABA(A) receptor-associated protein-like 1, Early estrogen-regulated protein, ATG8L, ATG8

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    WB analysis using 18721-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    WB analysis of mouse kidney using 18721-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    WB analysis of human heart using 18721-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    WB analysis of human spleen using 18721-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    IHC staining of human kidney using 18721-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    IHC staining of human kidney using 18721-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    IHC staining of mouse brain using 18721-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    IHC staining of mouse brain using 18721-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    FC experiment of HepG2 using 18721-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GABARAPL1-Specific Polyclonal antibody (18721-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse brain tissue, human heart tissue, human spleen tissue, mouse kidney tissue, mouse liver tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

18721-1-AP targets GABARAPL1-Specific in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name GABA(A) receptor-associated protein like 1
Calculated molecular weight 14 kDa
Observed molecular weight 17 kDa
GenBank accession numberNM_031412
Gene symbol GABARAPL1
Gene ID (NCBI) 23710
RRIDAB_10597556
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle. And at very low levels in the thymus and small intestine. The antibody is specific to GABARAPL1 and doesn't recognize the recombinant GABARAP and GABARAPL2.

Protocols

Product Specific Protocols
WB protocol for GABARAPL1-Specific antibody 18721-1-APDownload protocol
IHC protocol for GABARAPL1-Specific antibody 18721-1-APDownload protocol
FC protocol for GABARAPL1-Specific antibody 18721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

GABARAPL1 antibodies: target one protein, get one free!

Authors - Le Grand Jaclyn Nicole JN
View Article
humanWB

Sci Rep

Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization.

Authors - Alexandra Boeske
View Article
humanWB

BMC Musculoskelet Disord

MiR-145 inhibits the differentiation and proliferation of bone marrow stromal mesenchymal stem cells by GABARAPL1 in steroid-induced femoral head necrosis

Authors - Pengfei Xu
View Article