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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GABARAPL1 Monoclonal antibody, PBS Only

GABARAPL1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E10F6

Cat no : 66458-1-PBS

Synonyms

APG8 LIKE, APG8L, ATG8, ATG8L, GABARAPL1, GEC 1, GEC1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using GABARAPL1 Monoclonal antibody (66458-1-Ig)

    WB analysis of mouse brain using 66458-1-Ig (same clone as 66458-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using GABARAPL1 Monoclonal antibody (66458-1-Ig)

    WB analysis of rat brain using 66458-1-Ig (same clone as 66458-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using GABARAPL1 Monoclonal antibody (66458-1-Ig)

    WB analysis of pig brain using 66458-1-Ig (same clone as 66458-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66458-1-Ig (GABARAPL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GABARAPL1 Monoclonal antibody (66458-1-Ig)

    IF Staining of HepG2 using 66458-1-Ig (same clone as 66458-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GABARAPL1 antibody (66458-1-Ig, Clone: 1E10F6 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66458-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66458-1-PBS targets GABARAPL1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GABARAPL1 fusion protein Ag1473 相同性解析による交差性が予測される生物種
Full Name GABA(A) receptor-associated protein like 1
Calculated molecular weight 14 kDa
Observed molecular weight 16-18 kDa
GenBank accession numberBC009309
Gene symbol GABARAPL1
Gene ID (NCBI) 23710
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle and at very low levels in thymus and small intestine.