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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GABARAPL1-Specific Polyclonal antibody, PBS Only

GABARAPL1-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18721-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    WB analysis using 18721-1-AP (same clone as 18721-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    WB analysis of mouse kidney using 18721-1-AP (same clone as 18721-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    WB analysis of human heart using 18721-1-AP (same clone as 18721-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    WB analysis of human spleen using 18721-1-AP (same clone as 18721-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    IHC staining of human kidney using 18721-1-AP (same clone as 18721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    IHC staining of human kidney using 18721-1-AP (same clone as 18721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    IHC staining of mouse brain using 18721-1-AP (same clone as 18721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GABARAPL1-Specific Polyclonal antibody (18721-1-AP)

    IHC staining of mouse brain using 18721-1-AP (same clone as 18721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18721-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18721-1-PBS targets GABARAPL1-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name GABA(A) receptor-associated protein like 1
Calculated molecular weight 14 kDa
Observed molecular weight 17 kDa
GenBank accession numberNM_031412
Gene symbol GABARAPL1
Gene ID (NCBI) 23710
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. And at very low levels in thymus and small intestine.The antibody is specific to GABARAPL1, and it doesn't recognize the recombinant GABARAP and GABARAPL2.