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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using GABPB2 Polyclonal antibody (20870-1-AP)

    WB analysis of Jurkat using 20870-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GABPB2 Polyclonal antibody (20870-1-AP)

    WB analysis of HeLa using 20870-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using GABPB2 Polyclonal antibody (20870-1-AP)

    WB analysis of A375 using 20870-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GABPB2 Polyclonal antibody (20870-1-AP)

    WB analysis of HeLa using 20870-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GABPB2 Polyclonal antibody (20870-1-AP)

    WB analysis of HEK-293 using 20870-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20870-1-AP (GABPB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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GABPB2 Polyclonal antibody

GABPB2 Polyclonal Antibody for WB, ELISA
Cat No. 20870-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

GABPB-2, GABPB 2, GABP subunit beta-2, GABP subunit beta 2, GA-binding protein subunit beta-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, A375 cells, HEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20870-1-AP targets GABPB2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABPB2 fusion protein Ag14933 相同性解析による交差性が予測される生物種
Full Name GA binding protein transcription factor, beta subunit 2
Calculated molecular weight 448 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC027033
Gene Symbol GABPB2
Gene ID (NCBI) 126626
RRIDAB_10734321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TAK5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GABPB2 is a subunit of the GA-binding protein (GABP) transcription factor, which is a heterodimeric complex. The GABP complex plays a role in the regulation of gene transcription by binding to specific DNA sequences. GABPB2 specifically contributes to the transactivation of target genes (PMID: 18628204). The protein is primarily located in the nucleus. It is ubiquitously expressed in various tissues, including testis, ovary, and others. Studies have shown that GABPB2 is dispensable for normal lymphocyte development but moderately affects B cell responses (PMID: 18628204). Mice deficient in GABPB2 exhibit increased B cell proliferation and antibody production in response to certain stimuli. The molecular weight of GABPB2 is 48 kDa.

Protocols

Product Specific Protocols
WB protocol for GABPB2 antibody 20870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Mol Cell

Argininosuccinate lyase drives activation of mutant TERT promoter in glioblastomas

Authors - Zhumei Shi
  • KD Validated
View Article