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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GABRA2 Polyclonal antibody (28535-1-AP)

    WB analysis using 28535-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28535-1-AP (GABRA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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GABRA2 Polyclonal antibody

GABRA2 Polyclonal Antibody for WB, ELISA
Cat No. 28535-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28535-1-AP targets GABRA2 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABRA2 fusion protein Ag29301 相同性解析による交差性が予測される生物種
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 2
Calculated molecular weight 51 kDa
Observed molecular weight51 kDa
GenBank accession numberNM_000807
Gene Symbol GABRA2
Gene ID (NCBI) 2555
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP47869
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GABRA2 (gamma-aminobutyric acid type A receptor subunit alpha2), also known as DEE78. It is expected to be located in cell membrane, and the protein is highly expressed in brain. The protein is a ligand-gated chloride channel, which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:29961870). It also plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:29961870). The calculated molecular weight of GABRA2 is 51 kDa.

Protocols

Product Specific Protocols
WB protocol for GABRA2 antibody 28535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols