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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GABRA2 Recombinant antibody, PBS Only (Capture/Detector)

GABRA2 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230158E6

Cat no : 83057-2-PBS

Synonyms

230158E6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GABRA2 Recombinant antibody (83057-2-RR)

    WB analysis using 83057-2-RR (same clone as 83057-2-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83057-2-RR (GABRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83057-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GABRA2 Recombinant antibody (83057-2-RR)

    WB analysis using 83057-2-RR (same clone as 83057-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83057-2-RR (GABRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83057-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using GABRA2 Recombinant antibody (83057-2-RR)

    FC experiment of HeLa using 83057-2-RR (same clone as 83057-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GABRA2 Recombinant antibody (83057-2-RR, Clone:230158E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83057-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00067-2

    Cytometric bead array standard curve of MP00067-2

    Cytometric bead array standard curve of MP00067-2, GABRA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83057-3-PBS. Detection antibody: 83057-2-PBS. Standard: Ag29301. Range: 0.312-20 ng/mL.

  • Cytometric bead array standard curve of MP00067-3

    Cytometric bead array standard curve of MP00067-3

    Cytometric bead array standard curve of MP00067-3, GABRA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83057-2-PBS. Detection antibody: 83057-4-PBS. Standard: Ag29301. Range: 0.312-20 ng/mL.

  • Affinity and Kinetic Characterization of 83057-2-PBS

    Affinity and Kinetic Characterization of 83057-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83057-2-PBS against Human GABRA2 was performed. The affinity constant is 0.62 nM.

  • Affinity and Kinetic Characterization of 83057-2-RR

    Affinity and Kinetic Characterization of 83057-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83057-2-RR against Human GABRA2 were performed. The affinity constant is 0.403 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83057-2-PBS targets GABRA2 as part of a matched antibody pair:

MP00067-2: 83057-3-PBS capture and 83057-2-PBS detection (validated in Cytometric bead array)

MP00067-3: 83057-2-PBS capture and 83057-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GABRA2 fusion protein Ag29301 相同性解析による交差性が予測される生物種
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 2
Calculated molecular weight 51 kDa
Observed molecular weight50-51 kDa
GenBank accession numberNM_000807
Gene symbol GABRA2
Gene ID (NCBI) 2555
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GABRA2 (gamma-aminobutyric acid type A receptor subunit alpha2), also known as DEE78. It is expected to be located in cell membrane, and the protein is highly expressed in brain. The protein is a ligand-gated chloride channel, which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:29961870). It also plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:29961870). The calculated molecular weight of GABRA2 is 51 kDa.