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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GABRA5 Polyclonal antibody

GABRA5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27929-1-AP

Synonyms

GABRA5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GABRA5 Polyclonal antibody (27929-1-AP)

    WB analysis using 27929-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27929-1-AP (GABRA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GABRA5 Polyclonal antibody (27929-1-AP)

    IHC staining of mouse brain using 27929-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27929-1-AP (GABRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GABRA5 Polyclonal antibody (27929-1-AP)

    IHC staining of mouse brain using 27929-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27929-1-AP (GABRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27929-1-AP targets GABRA5 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABRA5 fusion protein Ag27376 相同性解析による交差性が予測される生物種
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 5
Calculated molecular weight 462 aa, 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC113422
Gene symbol GABRA5
Gene ID (NCBI) 2558
RRIDAB_2923580
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GABRA5 antibody 27929-1-APDownload protocol
IHC protocol for GABRA5 antibody 27929-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols