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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GABRA5 Polyclonal antibody

GABRA5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27929-1-AP

Synonyms

GABRA5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GABRA5 Polyclonal antibody (27929-1-AP)

    WB analysis using 27929-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27929-1-AP (GABRA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GABRA5 Polyclonal antibody (27929-1-AP)

    IHC staining of mouse brain using 27929-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27929-1-AP (GABRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GABRA5 Polyclonal antibody (27929-1-AP)

    IHC staining of mouse brain using 27929-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27929-1-AP (GABRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27929-1-AP targets GABRA5 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABRA5 fusion protein Ag27376 相同性解析による交差性が予測される生物種
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 5
Calculated molecular weight 462 aa, 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC113422
Gene symbol GABRA5
Gene ID (NCBI) 2558
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GABRA5 antibody 27929-1-APDownload protocol
IHC protocol for GABRA5 antibody 27929-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols