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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GABRG2 Polyclonal antibody (14104-1-AP)

    WB analysis using 14104-1-AP (same clone as 14104-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14104-1-AP (GABRG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14104-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GABRG2 Polyclonal antibody (14104-1-AP)

    IP experiment of mouse brain using 14104-1-AP (same clone as 14104-1-PBS)

    IP result of anti-GABRG2 (IP:14104-1-AP, 4ug; Detection:14104-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 14104-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using GABRG2 Polyclonal antibody (14104-1-AP)

    IF Staining of mouse cerebellum using 14104-1-AP (same clone as 14104-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using GABRG2 antibody (14104-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14104-1-PBS in a different storage buffer formulation.

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GABRG2 Polyclonal antibody, PBS Only

GABRG2 Polyclonal Antibody for WB, IF-P, IP, Indirect ELISA
Cat No. 14104-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14104-1-PBS targets GABRG2 in WB, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABRG2 fusion protein Ag5237 相同性解析による交差性が予測される生物種
Full Name gamma-aminobutyric acid (GABA) A receptor, gamma 2
Calculated molecular weight 467 aa, 54 kDa
Observed molecular weight 54-60 kDa
GenBank accession numberBC059389
Gene Symbol GABRG2
Gene ID (NCBI) 2566
RRIDAB_10693527
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18507
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GABRG2 (gamma-aminobutyric acid type A receptor subunit gamma2), which is expected to be located in cell membrane and expressed in brain and retina. The calculated molecular weight of the protein is 54 kDa. This antibody can recognize the 43 kDa band of target (PMID:12091434). And it also has glycosylation modification. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures.