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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GAC-specific Polyclonal antibody, PBS Only

GAC-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19958-1-PBS

Synonyms

GLS, KGA, GLS1, GAM, GAC

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GAC-specific Polyclonal antibody (19958-1-AP)

    WB analysis using 19958-1-AP (same clone as 19958-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19958-1-AP (GAC-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GAC-specific Polyclonal antibody (19958-1-AP)

    WB analysis of HEK-293 using 19958-1-AP (same clone as 19958-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19958-1-AP (GAC-specific Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using GAC-specific Polyclonal antibody (19958-1-AP)

    IP experiment of mouse kidney using 19958-1-AP (same clone as 19958-1-PBS)

    IP result of anti-GAC-specific (IP:19958-1-AP, 3ug; Detection:19958-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using GAC-specific Polyclonal antibody (19958-1-AP)

    IHC staining of human heart using 19958-1-AP (same clone as 19958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using GAC-specific Polyclonal antibody (19958-1-AP)

    IHC staining of human heart using 19958-1-AP (same clone as 19958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using GAC-specific Polyclonal antibody (19958-1-AP)

    IHC staining of human heart using 19958-1-AP (same clone as 19958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using GAC-specific Polyclonal antibody (19958-1-AP)

    IHC staining of human heart using 19958-1-AP (same clone as 19958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GAC-specific Polyclonal antibody (19958-1-AP)

    IHC staining of human kidney using 19958-1-AP (same clone as 19958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 19958-1-AP (GAC-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GAC-specific Polyclonal antibody (19958-1-AP)

    IHC staining of human kidney using 19958-1-AP (same clone as 19958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 19958-1-AP (GAC-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GAC-specific Polyclonal antibody (19958-1-AP)

    IHC staining of human pancreas cancer using 19958-1-AP (same clone as 19958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GAC-specific Polyclonal antibody (19958-1-AP)

    IHC staining of human pancreas cancer using 19958-1-AP (same clone as 19958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using GAC-specific Polyclonal antibody (19958-1-AP)

    IF Staining of HEK-293 using 19958-1-AP (same clone as 19958-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GAC-specific antibody (19958-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 19958-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19958-1-PBS targets GAC-specific in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name glutaminase
Calculated molecular weight 65 kDa
Observed molecular weight 58 kDa
GenBank accession numberAF158555
Gene symbol GLS
Gene ID (NCBI) 2744
RRIDAB_10640899
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glutaminase C (GAC), a splicing variant of the kidney-type glutaminase (KGA) gene, is a vital mitochondrial enzyme protein that catalyzes glutamine to glutamate. GAC is the only isoform present in the mitochondria (PMID:22228304). In a normal state, immunodecorationwith antibodies directed against GAC identified an abundant complex of 232 kDa as a tetrameric form of GAC, the DPAA can directly destructed the native tetrameric GAC complex to accumulate the unassembled one (50-90 kDa). (PMID:22493432). This antibody is specific to GAC isoform. It has no cross reaction to KGA.