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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAD65 Polyclonal antibody (20746-1-AP)

    WB analysis using 20746-1-AP (same clone as 20746-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20746-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using GAD65 Polyclonal antibody (20746-1-AP)

    WB analysis of mouse brain using 20746-1-AP (same clone as 20746-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20746-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using GAD65 Polyclonal antibody (20746-1-AP)

    WB analysis of mouse brain using 20746-1-AP (same clone as 20746-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20746-1-AP (GAD65 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20746-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GAD65 Polyclonal antibody (20746-1-AP)

    IP experiment of mouse brain using 20746-1-AP (same clone as 20746-1-PBS)

    IP result of anti-GAD65 (IP:20746-1-AP, 4ug; Detection:20746-1-AP 1:1000) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 20746-1-PBS in a different storage buffer formulation.

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GAD65 Polyclonal antibody, PBS Only

GAD65 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 20746-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20746-1-PBS targets GAD65 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberNM_000818
Gene Symbol GAD65
Gene ID (NCBI) 2572
RRIDAB_10949196
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05329
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GAD2, also named as GAD65, belongs to the group II decarboxylase family. GAD2 catalyzes the production of GABA. It is responsible for the synthesis of the essential neurotransmitter gamma-aminobutyric acid (GABA) from L-glutamic acid. GAD2 is expressed in nervous and endocrine systems and are thought to be involved in synaptic transmission and INS secretion. Autoantibodies against GAD2 may serve as markers for type I diabetes. Many individuals suffering from an adult onset disorder known as Stiff Person Syndrome (SPS) also express autoantibodies to GAD2. The antibody is specific to GAD2.