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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GADD34 Recombinant antibody

GADD34 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4K3

Cat no : 81250-1-RR

Synonyms

PPP1R15A, Growth arrest and DNA damage-inducible protein GADD34, growth arrest and DNA damage-inducible protein 34, 4K3

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using GADD34 Recombinant antibody (81250-1-RR)

    WB analysis of NIH/3T3 using 81250-1-RR

    MG132 treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81250-1-RR (GADD34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GADD34 Recombinant antibody (81250-1-RR)

    WB analysis of HeLa using 81250-1-RR

    MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 81250-1-RR (GADD34 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GADD34 Recombinant antibody (81250-1-RR)

    WB analysis of HepG2 using 81250-1-RR

    MG132 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 81250-1-RR (GADD34 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using GADD34 Recombinant antibody (81250-1-RR)

    IF Staining of PC-3 using 81250-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GADD34 antibody (81250-1-RR, Clone: 4K3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 81250-1-RR

    Affinity and Kinetic Characterization of 81250-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81250-1-RR against Human GADD34 were performed. The affinity constant is 93.8 pM.

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Tested Applications

Positive WB detected inMG132 treated HepG2 cells, MG132 treated NIH/3T3 cells, MG132 treated HeLa cells
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81250-1-RR targets GADD34 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GADD34 fusion protein Ag0578 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 15A
Calculated molecular weight 73 kDa
Observed molecular weight100 kDa
GenBank accession numberBC003067
Gene symbol GADD34
Gene ID (NCBI) 23645
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 is 100 kDa confirmed in GADD34 KO mice, and Proteintech's GADD34 antibody 81250-1-RR primarily recongizes the 100 kDa band.

Protocols

Product Specific Protocols
WB protocol for GADD34 antibody 81250-1-RRDownload protocol
IF protocol for GADD34 antibody 81250-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols