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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GADD45A Recombinant antibody

GADD45A Recombinant Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241597F5

Cat no : 84376-3-RR

Synonyms

GADD45, DNA damage-inducible transcript 1 protein, DDIT-1, DDIT1, DDIT 1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GADD45A Recombinant antibody (84376-3-RR)

    IHC staining of human stomach cancer using 84376-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84376-3-RR (GADD45A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GADD45A Recombinant antibody (84376-3-RR)

    IHC staining of human stomach cancer using 84376-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84376-3-RR (GADD45A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using GADD45A Recombinant antibody (84376-3-RR)

    IF Staining of U2OS using 84376-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GADD45A antibody (84376-3-RR, Clone: 241597F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84376-3-RR

    Affinity and Kinetic Characterization of 84376-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84376-3-RR against Human GADD45A were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84376-3-RR is GADD45A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GADD45A fusion protein Ag4687 相同性解析による交差性が予測される生物種
Full Name growth arrest and DNA-damage-inducible, alpha
Calculated molecular weight 165 aa, 18 kDa
GenBank accession numberBC011757
Gene symbol GADD45A
Gene ID (NCBI) 1647
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for GADD45A antibody 84376-3-RRDownload protocol
IF protocol for GADD45A antibody 84376-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols