GADD45GIP1 Polyclonal antibody

GADD45GIP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA, IF

Conjugate

Unconjugated

Cat no : 16260-1-AP

Synonyms

CKBBP2, CKII beta associating protein, CR6 interacting factor 1, CRIF1, GADD45GIP1, p53 responsive gene 6 protein, PLINP 1, Plinp1, PRG6


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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, mouse heart tissue, HEK-293 cells, SKOV-3 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16260-1-AP targets GADD45GIP1 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GADD45GIP1 fusion protein Ag9299 相同性解析による交差性が予測される生物種
Full Name growth arrest and DNA-damage-inducible, gamma interacting protein 1
Calculated molecular weight 222 aa, 25 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC013027
Gene symbol GADD45GIP1
Gene ID (NCBI) 90480
RRIDAB_2108229
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GADD45GIP1 antibody 16260-1-APDownload protocol
IHC protocol for GADD45GIP1 antibody 16260-1-APDownload protocol
IP protocol for GADD45GIP1 antibody 16260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Science

T cell metabolism. The protein LEM promotes CD8⁺ T cell immunity through effects on mitochondrial respiration.

Authors - Isobel Okoye
humanIHC

Oncotarget

CR6-interacting factor 1 inhibits invasiveness by suppressing TGF-β-mediated epithelial-mesenchymal transition in hepatocellular carcinoma.

Authors - Runzhou Zhuang
ratWB

J Mol Med (Berl)

Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury.

Authors - Li Peng
mouseWB

Burns Trauma

Cardiac proteomics reveals the potential mechanism of microtubule associated protein 4 phosphorylation-induced mitochondrial dysfunction.

Authors - Lingfei Li
mouseWB,IHC

Int J Mol Sci

Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1

Authors - Kazuhiro Hasegawa