Validation Data Gallery
Tested Applications
| Positive WB detected in | SKOV-3 cells, Jurkat cells, U-251 cells |
| Positive IHC detected in | human ovarian cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83823-5-RR targets GADD45GIP1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | GADD45GIP1 fusion protein Ag9299 相同性解析による交差性が予測される生物種 |
| Full Name | growth arrest and DNA-damage-inducible, gamma interacting protein 1 |
| Calculated molecular weight | 222 aa, 25 kDa |
| Observed molecular weight | 25-28 kDa |
| GenBank accession number | BC013027 |
| Gene Symbol | GADD45GIP1 |
| Gene ID (NCBI) | 90480 |
| RRID | AB_3671407 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q8TAE8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Growth arrest and DNA-damage-inducible proteins-interacting protein 1(GADD45GIP1), also known as CRIF1, is newly identified de novo components in large subunit of mitoribosome. It is essential for the translation of mitochondrial oxidative phosphorylation (OXPHOS) polypeptides in mammalian mitochondria. The essential role of CRIF1 in mitochondrial synthesis and membrane integration of OXPHOS polypeptides was shown in brain-specific CRIF1-deficient mice, which exhibited profound OXPHOS failure and marked neurodegeneration. (PMID 22819524) In addition, GADD45GIP1 enhances the function of GADD45 in maintaining genome stability, DNA repair and cell cycle regulation by interacting with the GADD45 protein family. It's reported that GADD45GIP1 may be induced by p53, a process that is closely related to the cell's response to DNA damage.(PMID:28599472)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GADD45GIP1 antibody 83823-5-RR | Download protocol |
| IHC protocol for GADD45GIP1 antibody 83823-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |