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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GAGE1 Polyclonal antibody

GAGE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12795-1-AP

Synonyms

Cancer/testis antigen 4.4, CT4.1, CT4.4, G antigen 1, G antigen 4, GAGE 4, GAGE1, GAGE4

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using GAGE1 Polyclonal antibody (12795-1-AP)

    WB analysis of Jurkat using 12795-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12795-1-AP (GAGE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GAGE1 Polyclonal antibody (12795-1-AP)

    IHC staining of human lung cancer using 12795-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12795-1-AP (GAGE1 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12795-1-AP targets GAGE1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAGE1 fusion protein Ag3534 相同性解析による交差性が予測される生物種
Full Name G antigen 1
Calculated molecular weight 139 aa, 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC036094
Gene symbol GAGE1
Gene ID (NCBI) 2543
RRIDAB_2108363
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GAGE1 antibody 12795-1-APDownload protocol
IHC protocol for GAGE1 antibody 12795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols