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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using GAGE2D Polyclonal antibody (12532-1-AP)

    WB analysis of K-562 using 12532-1-AP (same clone as 12532-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using GAGE2D Polyclonal antibody (12532-1-AP)

    WB analysis of human testis using 12532-1-AP (same clone as 12532-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GAGE2D Polyclonal antibody (12532-1-AP)

    WB analysis of HeLa using 12532-1-AP (same clone as 12532-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GAGE2D Polyclonal antibody (12532-1-AP)

    IHC staining of human liver cancer using 12532-1-AP (same clone as 12532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GAGE2D Polyclonal antibody (12532-1-AP)

    IHC staining of human liver cancer using 12532-1-AP (same clone as 12532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using GAGE2D Polyclonal antibody (12532-1-AP)

    IHC staining of human testis using 12532-1-AP (same clone as 12532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using GAGE2D Polyclonal antibody (12532-1-AP)

    IHC staining of human testis using 12532-1-AP (same clone as 12532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GAGE2D Polyclonal antibody (12532-1-AP)

    IF Staining of HepG2 using 12532-1-AP (same clone as 12532-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using GAGE2D antibody 12532-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12532-1-PBS in a different storage buffer formulation.

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GAGE2D Polyclonal antibody, PBS Only

GAGE2D Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12532-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12532-1-PBS targets GAGE2D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAGE2D fusion protein Ag3230 相同性解析による交差性が予測される生物種
Full Name G antigen 2D
Calculated molecular weight 116 aa, 13 kDa
Observed molecular weight 18-22 kDa
GenBank accession numberBC018052
Gene Symbol GAGE2D
Gene ID (NCBI) 729408
RRIDAB_2877864
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UEU5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GAGE2D, also named as GAGE8 and CT4.8, is expressed in testis and tumor tissues.