Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HeLa cells using 14979-1-AP
WB result of Galectin 3 antibody (14979-1-AP, 1:1000) with si-Control and si-Galectin 3 transfected HeLa cells.
WB analysis using 14979-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14979-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat colon using 14979-1-AP
rat colon tissue were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 14979-1-AP
IP result of anti-Galectin-3 (IP:14979-1-AP, 3ug; Detection:14979-1-AP 1:500) with MCF-7 cells lysate 1600ug.
IHC staining of human thyroid cancer using 14979-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP (Galectin-3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 14979-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP (Galectin-3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 14979-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14979-1-AP (Galectin-3 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14979-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Galectin-3 antibody (14979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HeLa using 14979-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.4 ug Galectin-3 Polyclonal antibody (14979-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

Galectin-3 Polyclonal antibody

Galectin-3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14979-1-AP

Publications(41)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

GAL3, Gal 3, CBP35, CBP 35, Carbohydrate-binding protein 35

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (41)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, human heart tissue, rat colon tissue, MCF-7 cells, NIH/3T3 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human thyroid cancer tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 22 publications below
IHC	See 12 publications below
IF	See 17 publications below
IP	See 2 publications below

Product Information

14979-1-AP targets Galectin-3 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Galectin-3 fusion protein Ag6891 相同性解析による交差性が予測される生物種
Full Name	lectin, galactoside-binding, soluble, 3
Calculated molecular weight	26 kDa
Observed molecular weight	31 kDa
GenBank accession number	BC001120
Gene Symbol	Galectin-3
Gene ID (NCBI)	3958
RRID	AB_2136768
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P17931
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

Protocols

Product Specific Protocols
WB protocol for Galectin-3 antibody 14979-1-AP	Download protocol
IHC protocol for Galectin-3 antibody 14979-1-AP	Download protocol
IF protocol for Galectin-3 antibody 14979-1-AP	Download protocol
IP protocol for Galectin-3 antibody 14979-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nature A phosphoinositide signalling pathway mediates rapid lysosomal repair Authors - Jay Xiaojun Tan View Article
mouse	WB	Nat Immunol Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions. Authors - Rui Martins View Article
mouse	IF	Redox Biol Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury Authors - Jing Yu View Article
mouse	IF	Br J Pharmacol Substitution of the SERCA2 Cys(674) reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation Authors - Hang Su View Article
human	IF	J Neuroinflammation Progranulin haploinsufficiency mediates cytoplasmic TDP-43 aggregation with lysosomal abnormalities in human microglia Authors - Wonjae Sung View Article
human,mouse	WB	Front Cell Dev Biol Activation of Galectin-3 (LGALS3) Transcription by Injurious Stimuli in the Liver Is Commonly Mediated by BRG1. Authors - Zilong Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa cells using 14979-1-AP

WB analysis using 14979-1-AP

WB analysis of human heart using 14979-1-AP

WB analysis of rat colon using 14979-1-AP

IP experiment of MCF-7 using 14979-1-AP

IHC staining of human thyroid cancer using 14979-1-AP

IHC staining of human thyroid cancer using 14979-1-AP

IHC staining of human colon using 14979-1-AP

IF Staining of HeLa using 14979-1-AP

FC experiment of HeLa using 14979-1-AP

Galectin-3 Polyclonal antibody

Galectin-3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.

Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury

Substitution of the SERCA2 Cys(674) reactive thiol accelerates atherosclerosis by inducing endoplasmic reticulum stress and inflammation

Progranulin haploinsufficiency mediates cytoplasmic TDP-43 aggregation with lysosomal abnormalities in human microglia

Activation of Galectin-3 (LGALS3) Transcription by Injurious Stimuli in the Liver Is Commonly Mediated by BRG1.