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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GALK1 Polyclonal antibody (15284-1-AP)

    WB analysis using 15284-1-AP (same clone as 15284-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15284-1-AP (GALK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15284-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using GALK1 Polyclonal antibody (15284-1-AP)

    WB analysis of mouse heart using 15284-1-AP (same clone as 15284-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15284-1-AP (GALK1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15284-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GALK1 Polyclonal antibody (15284-1-AP)

    IHC staining of human breast cancer using 15284-1-AP (same clone as 15284-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15284-1-AP (GALK1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15284-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GALK1 Polyclonal antibody (15284-1-AP)

    IF Staining of MCF-7 using 15284-1-AP (same clone as 15284-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 15284-1-AP (GALK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15284-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GALK1 Polyclonal antibody, PBS Only

GALK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15284-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15284-1-PBS targets GALK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GALK1 fusion protein Ag7403 相同性解析による交差性が予測される生物種
Full Name galactokinase 1
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC001166
Gene Symbol GALK1
Gene ID (NCBI) 2584
RRIDAB_2108553
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51570
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GALK1, also named as GALK, belongs to the GHMP kinase family and GalK subfamily. GALK1, located on chromosome 17q24, encodes galactokinase and is involved in galactose metabolism (PMID: 32807972). The molecular weight of GALK1 is 42 kDa.