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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GALNT6 Polyclonal antibody (13671-1-AP)

    WB analysis using 13671-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13671-1-AP (GALNT6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GALNT6 Polyclonal antibody (13671-1-AP)

    IF Staining of A431 using 13671-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GALNT6 antibody (13671-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

GALNT6 Polyclonal antibody

GALNT6 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13671-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

pp GaNTase 6, Polypeptide N-acetylgalactosaminyltransferase 6, GalNAc-T6, GalNAcT6, GALNAC T6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, MCF-7 cells, T-47D cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13671-1-AP targets GALNT6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GALNT6 fusion protein Ag4652 相同性解析による交差性が予測される生物種
Full Name UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 (GalNAc-T6)
Calculated molecular weight 622 aa, 71 kDa
Observed molecular weight71 kDa
GenBank accession numberBC035822
Gene Symbol GALNT6
Gene ID (NCBI) 11226
RRIDAB_3669168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NCL4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GALNT6 (polypeptide N-acetylgalactosaminyltransferase 6, GalNAc-T6), which is involved in the initiation of O-glycosylation, has been reported to play crucial roles in mammary carcinogenesis through binding to several substrates (PMID: 32393740). GALNT6 is upregulated in breast, ovarian, renal cell, lung and pancreatic cancers (PMID: 20215525). Overexpression of GRP78 could enhance Golgi-to-ER relocation of GALNT6 (PMID: 28110670).

Protocols

Product Specific Protocols
WB protocol for GALNT6 antibody 13671-1-APDownload protocol
IF protocol for GALNT6 antibody 13671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols