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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using GALT Polyclonal antibody (17035-1-AP)

    WB analysis of human liver using 17035-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17035-1-AP (GALT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using GALT Polyclonal antibody (17035-1-AP)

    WB analysis of human liver using 17035-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17035-1-AP (GALT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using GALT Polyclonal antibody (17035-1-AP)

    IHC staining of human liver using 17035-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17035-1-AP (GALT antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using GALT Polyclonal antibody (17035-1-AP)

    IHC staining of human liver using 17035-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17035-1-AP (GALT antibody) at dilution of 1:100 (under 40x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

GALT Polyclonal antibody

GALT Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17035-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Gal 1 P uridylyltransferase, GALT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

17035-1-AP targets GALT in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GALT fusion protein Ag10524 相同性解析による交差性が予測される生物種
Full Name galactose-1-phosphate uridylyltransferase
Calculated molecular weight 379 aa, 43 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC015045
Gene Symbol GALT
Gene ID (NCBI) 2592
RRIDAB_2109296
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07902
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GALT (galactose-1-phosphate uridylyltransferase) catalyses the second step in the Leloir pathway for the conversion of galactose-l-phosphate (Gal-I-P) into uridine diphosphogalactose (UDP-gal). Defects in GALT cause galactosemia.

Protocols

Product Specific Protocols
WB protocol for GALT antibody 17035-1-APDownload protocol
IHC protocol for GALT antibody 17035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Inherit Metab Dis

Galactose 1-phosphate accumulates to high levels in galactose-treated cells due to low GALT activity and absence of product inhibition of GALK.

Authors - Sher Li Oh
View Article
humanWB

PLoS One

Angelica Sinensis Polysaccharides Stimulated UDP-Sugar Synthase Genes through Promoting Gene Expression of IGF-1 and IGF1R in Chondrocytes: Promoting Anti-Osteoarthritic Activity.

Authors - Yinxian Wen
View Article