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  • Featured Product
  • KD/KO Validated

GANAB Polyclonal antibody

GANAB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29183-1-AP

Synonyms

glucosidase, alpha; neutral AB, Glucosidase II subunit alpha, G2AN, EC:3.2.1.207, Alpha-glucosidase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GANAB Polyclonal antibody (29183-1-AP)

    WB analysis using 29183-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29183-1-AP (GANAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GANAB Polyclonal antibody (29183-1-AP)

    WB analysis of HeLa using 29183-1-AP

    WB result of GANAB antibody (29183-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GANAB transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human colon tissue using GANAB Polyclonal antibody (29183-1-AP)

    IHC staining of human colon using 29183-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 29183-1-AP (GANAB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GANAB Polyclonal antibody (29183-1-AP)

    IHC staining of mouse liver using 29183-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29183-1-AP (GANAB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GANAB Polyclonal antibody (29183-1-AP)

    IHC staining of mouse liver using 29183-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29183-1-AP (GANAB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse kidney tissue, HeLa cells, mouse liver, rat liver
Positive IHC detected inmouse liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29183-1-AP targets GANAB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GANAB fusion protein Ag30417 相同性解析による交差性が予測される生物種
Full Name glucosidase, alpha; neutral AB
Calculated molecular weight944aa,107 kDa; 488aa,55 kDa
Observed molecular weight110-120 kDa
GenBank accession numberBC017435
Gene symbol GANAB
Gene ID (NCBI) 23193
RRIDAB_2918244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GANAB antibody 29183-1-APDownload protocol
IHC protocol for GANAB antibody 29183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Glycobiology

MeCP2 regulated glycogenes contribute to proliferation and apoptosis of gastric cancer cells.

Authors - Yannan Qin
  • KD Validated
View Article