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GANAB Recombinant antibody

GANAB Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240279B7

Cat no : 83324-1-RR

Synonyms

Glucosidase II subunit alpha, G2AN, EC:3.2.1.207, Alpha-glucosidase 2, Alpha glucosidase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GANAB Recombinant antibody (83324-1-RR)

    WB analysis using 83324-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83324-1-RR (GANAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GANAB Recombinant antibody (83324-1-RR)

    WB analysis of HeLa using 83324-1-RR

    WB result of GANAB antibody (83324-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GANAB transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GANAB Recombinant antibody (83324-1-RR)

    IHC staining of mouse liver using 83324-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83324-1-RR (GANAB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GANAB Recombinant antibody (83324-1-RR)

    IF Staining of HeLa using 83324-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GANAB antibody (83324-1-RR, Clone: 240279B7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using GANAB Recombinant antibody (83324-1-RR)

    FC experiment of HeLa using 83324-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GANAB Recombinant antibody (83324-1-RR, Clone:240279B7)(red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83324-1-RR

    Affinity and Kinetic Characterization of 83324-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83324-1-RR against Human GANAB were performed. The affinity constant is 0.186 nM.

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Tested Applications

Positive WB detected inHeLa cells, A431 cells, HepG2 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue, human placenta tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83324-1-RR is GANAB Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GANAB fusion protein Ag30417 相同性解析による交差性が予測される生物種
Full Name glucosidase, alpha; neutral AB
Calculated molecular weight944aa,107 kDa; 488aa,55 kDa
Observed molecular weight110 kDa
GenBank accession numberBC017435
Gene symbol GANAB
Gene ID (NCBI) 23193
RRIDAB_3670990
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GANAB antibody 83324-1-RRDownload protocol
IHC protocol for GANAB antibody 83324-1-RRDownload protocol
IF protocol for GANAB antibody 83324-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols