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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAPDH (Human Specific) Recombinant antibody (80570-1-RR)

    WB analysis using 80570-1-RR (same clone as 80570-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80570-1-RR (GAPDH antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GAPDH (Human Specific) Recombinant antibody (80570-1-RR)

    IHC staining of human liver cancer using 80570-1-RR (same clone as 80570-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GAPDH (Human Specific) Recombinant antibody (80570-1-RR)

    IHC staining of human liver cancer using 80570-1-RR (same clone as 80570-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using GAPDH (Human Specific) Recombinant antibody (80570-1-RR)

    IHC staining of human lung using 80570-1-RR (same clone as 80570-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using GAPDH (Human Specific) Recombinant antibody (80570-1-RR)

    IHC staining of human lung using 80570-1-RR (same clone as 80570-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80570-1-RR (GAPDH (Human Specific) antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GAPDH (Human Specific) Recombinant antibody (80570-1-RR)

    IF Staining of HeLa using 80570-1-RR (same clone as 80570-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH (Human Specific) antibody (80570-1-RR, Clone: 2G21 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GAPDH (Human Specific) Recombinant antibody (80570-1-RR)

    IF Staining of HeLa using 80570-1-RR (same clone as 80570-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH (Human Specific) antibody (80570-1-RR, Clone: 2G21 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80570-1-PBS in a different storage buffer formulation.

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GAPDH (Human Specific) Recombinant antibody, PBS Only

GAPDH (Human Specific) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 80570-1-PBS
Clone No.2G21

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80570-1-PBS targets GAPDH (Human Specific) in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GAPDH (Human Specific) fusion protein Ag0766 相同性解析による交差性が予測される生物種
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight 36 kDa
GenBank accession numberBC004109
Gene Symbol GAPDH
Gene ID (NCBI) 2597
RRIDAB_2918902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04406
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable. 80570-1-RR is human specific.