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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GAPVD1 Polyclonal antibody (16940-1-AP)

    WB analysis using 16940-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16940-1-AP (GAPVD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using GAPVD1 Polyclonal antibody (16940-1-AP)

    IHC staining of human placenta using 16940-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16940-1-AP (GAPVD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using GAPVD1 Polyclonal antibody (16940-1-AP)

    IHC staining of human placenta using 16940-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16940-1-AP (GAPVD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GAPVD1 Polyclonal antibody (16940-1-AP)

    IF Staining of A431 using 16940-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GAPVD1 antibody (16940-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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GAPVD1 Polyclonal antibody

GAPVD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16940-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RAP6, Rab5-activating protein 6, KIAA1521, GTPase-activating protein and VPS9 domain-containing protein 1, GAPex-5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16940-1-AP targets GAPVD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAPVD1 fusion protein Ag10492 相同性解析による交差性が予測される生物種
Full Name GTPase activating protein and VPS9 domains 1
Calculated molecular weight 1478 aa, 165 kDa
Observed molecular weight180-200 kDa
GenBank accession numberBC021119
Gene Symbol GAPVD1
Gene ID (NCBI) 26130
RRIDAB_3085511
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14C86
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GTPase activating protein and VPS9 domains 1(GAPVD1) is a multifunctional protein involved in cellular processes such as endocytosis, insulin receptor internalization, and GLUT4 trafficking. Its function is critical in maintaining cellular homeostasis and it interacts with various proteins, including those involved in endocytosis and circadian rhythm regulation.

Protocols

Product Specific Protocols
WB protocol for GAPVD1 antibody 16940-1-APDownload protocol
IHC protocol for GAPVD1 antibody 16940-1-APDownload protocol
IF protocol for GAPVD1 antibody 16940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols