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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GAR1 Recombinant antibody, PBS Only (Detector)

GAR1 Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240537C11

Cat no : 83577-1-PBS

Synonyms

snoRNP protein GAR1, NOLA1, 240537C11

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GAR1 Recombinant antibody (83577-1-RR)

    IF Staining of HepG2 using 83577-1-RR (same clone as 83577-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GAR1 antibody (83577-1-RR, Clone: 240537C11 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K562 using GAR1 Recombinant antibody (83577-1-RR)

    FC experiment of K562 using 83577-1-RR (same clone as 83577-1-PBS)

    1x10^6 K562 were intracellularly stained with 0.25 ug GAR1 Recombinant antibody (83577-1-RR, Clone:240537C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00568-1

    Cytometric bead array standard curve of MP00568-1

    Cytometric bead array standard curve of MP00568-1, GAR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83577-2-PBS. Detection antibody: 83577-1-PBS. Standard: Ag2282. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP00568-2

    Cytometric bead array standard curve of MP00568-2

    Cytometric bead array standard curve of MP00568-2, GAR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83577-3-PBS. Detection antibody: 83577-1-PBS. Standard: Ag2282. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83577-1-RR

    Affinity and Kinetic Characterization of 83577-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83577-1-RR against Human GAR1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83577-1-PBS targets GAR1 as part of a matched antibody pair:

MP00568-1: 83577-2-PBS capture and 83577-1-PBS detection (validated in Cytometric bead array)

MP00568-2: 83577-3-PBS capture and 83577-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GAR1 fusion protein Ag2282 相同性解析による交差性が予測される生物種
Full Name GAR1 ribonucleoprotein homolog (yeast)
Calculated molecular weight 217 aa, 22 kDa
GenBank accession numberBC003413
Gene symbol GAR1
Gene ID (NCBI) 54433
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.