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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GARNL1 Recombinant antibody (82971-1-RR)

    WB analysis using 82971-1-RR (same clone as 82971-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82971-1-RR (GARNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GARNL1 Recombinant antibody (82971-1-RR)

    WB analysis of HEK-293 using 82971-1-RR (same clone as 82971-1-PBS)

    WB result of GARNL1 antibody (82971-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARNL1 transfected HEK-293 cells. This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using GARNL1 Recombinant antibody (82971-1-RR)

    FC experiment of U2OS using 82971-1-RR (same clone as 82971-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug GARNL1 Recombinant antibody (82971-1-RR, Clone:230165F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82971-1-RR

    Affinity and Kinetic Characterization of 82971-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82971-1-RR against Human GARNL1 were performed. The affinity constant is 99.6 pM.

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  • Featured Product
  • KD/KO Validated

GARNL1 Recombinant antibody, PBS Only

GARNL1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82971-1-PBS
Clone No.230165F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82971-1-PBS targets GARNL1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GARNL1 fusion protein Ag31518 相同性解析による交差性が予測される生物種
Full Name GTPase activating Rap/RanGAP domain-like 1
Calculated molecular weight230KD
Observed molecular weight260 kDa
GenBank accession numberNM_014990.3
Gene Symbol GARNL1
Gene ID (NCBI) 253959
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6GYQ0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, is a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).