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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RAP1GAP2 Polyclonal antibody

RAP1GAP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25391-1-AP

Synonyms

GARNL4, RAP1GA3, RAP1GA2, Rap1 GTPase-activating protein 2, KIAA1039

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAP1GAP2 Polyclonal antibody (25391-1-AP)

    WB analysis using 25391-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25391-1-AP (GARNL4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RAP1GAP2 Polyclonal antibody (25391-1-AP)

    IHC staining of human stomach cancer using 25391-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25391-1-AP (RAP1GAP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25391-1-AP targets RAP1GAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAP1GAP2 fusion protein Ag21840 相同性解析による交差性が予測される生物種
Full Name GTPase activating Rap/RanGAP domain-like 4
Calculated molecular weight 730 aa, 80 kDa
Observed molecular weight80 kDa
GenBank accession numberBC140870
Gene symbol RAP1GAP2
Gene ID (NCBI) 23108
RRIDAB_2880057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RAP1GAP2 antibody 25391-1-APDownload protocol
IHC protocol for RAP1GAP2 antibody 25391-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols