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GARS Polyclonal antibody
GARS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 15831-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human brain tissue, HeLa cells, HepG2 cells, HT-1080 cells |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 9 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
CoIP | See 2 publications below |
RIP | See 1 publications below |
Product Information
15831-1-AP targets GARS in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, Drosophila |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GARS fusion protein Ag8322 相同性解析による交差性が予測される生物種 |
Full Name | glycyl-tRNA synthetase |
Calculated molecular weight | 739 aa, 83 kDa |
Observed molecular weight | 75-80 kDa |
GenBank accession number | BC007755 |
Gene symbol | GARS |
Gene ID (NCBI) | 2617 |
RRID | AB_2294385 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GARS(glycinamide ribonucleotide synthetase) perform an essential function in protein synthesis by catalyzing the esterification of an amino acid to its cognate tRNA.GARS, however, is a ubiquitously expressed α2 homodimer responsible for charging tRNA molecules with glycine, this enzyme is essential for protein synthesis in all cells and tissues(PMID:17035524 ).It can exist as a oligomer with 250KDa(PMID:22504945).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for GARS antibody 15831-1-AP | Download protocol |
IHC protocol for GARS antibody 15831-1-AP | Download protocol |
IF protocol for GARS antibody 15831-1-AP | Download protocol |
IP protocol for GARS antibody 15831-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules | ||
J Hematol Oncol Integrated proteogenomic characterization of urothelial carcinoma of the bladder. | ||
Brain HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease.
| ||
Nat Commun Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases. | ||
EMBO Rep The tRNA-like small noncoding RNA mascRNA promotes global protein translation.
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